Soluble DHFR expression ranges in molecules for every cell calculated from lysate exercise assays as explained in Materials and approaches.
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three% of all solitary issue mutations in DHFR are useful within the absence of Lon, but useful mutations are largely suppressed when Lon is reintroduced. Protein stability measurements display substantial exercise-stability tradeoffs with the advantageous mutants and provide a mechanistic clarification for Lon’s common effect. Our conclusions propose prospects for tuning mutational landscapes by modulating the cellular natural environment, with implications for protein structure and combatting antibiotic resistance.
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